国产精品久久久久久AV福利,日本丰满少妇XXXX,无码国产精品一区二区免费式影视,国产精品免费看久久久无码

您好,歡迎進(jìn)入上海力敏實(shí)業(yè)有限公司網(wǎng)站!
一鍵分享網(wǎng)站到:
您現(xiàn)在的位置:首頁(yè) >> 產(chǎn)品中心 >> 生化試劑、抗體、血清 >> 常規(guī)生化試劑 >> qiagen301427Effectene Transfection Reagent effectene轉(zhuǎn)染試劑

Effectene Transfection Reagent effectene轉(zhuǎn)染試劑

  • 更新時(shí)間:  2023-07-24
  • 產(chǎn)品型號(hào):  qiagen301427
  • 簡(jiǎn)單描述
  • Effectene Transfection Reagent effectene轉(zhuǎn)染試劑
    對(duì)各種類型的真核細(xì)胞DNA轉(zhuǎn)染,尤其適用于原代細(xì)胞
    可在血清存在的情況下進(jìn)行轉(zhuǎn)染
    適合用于原代細(xì)胞或敏感的細(xì)胞系
    高轉(zhuǎn)染效率而且較低的DNA使用量
    Low endotoxin level with <10 EU/ml
詳細(xì)介紹

Effectene Transfection Reagent  effectene轉(zhuǎn)染試劑

Principle

Effectene Transfection Reagent is an innovative non-liposomal lipid formulation that is used in conjunction with a special DNA-condensing enhancer and optimized buffer to achieve high transfection efficiencies. The enhancer first condenses the DNA molecules and Effectene Reagent subsequently coats them with cationic lipids (see figure "Model of Effectene Principle"), providing a particularly efficient way of transferring DNA into eukaryotic cells. Effectene Reagent offers significant advantages over many liposome reagents and other transfection methods (1, 2).

Procedure

The Effectene procedure has two steps. DNA is first mixed with Enhancer and a buffer that provides optimal salt conditions for efficient DNA condensation. This step requires just 2–5 minutes. Effectene Reagent is then added and the mixture is incubated for 5–10 minutes to allow Effectene–DNA complexes to form. The complexes are mixed with growth medium (which can contain serum and antibiotics), and added directly to the cells. The cells are then incubated until harvested and analyzed for gene expression (see flowchart "Effectene Transfection Reagent").

Applications

Effectene Reagent is suitable for transient and stable transfection of a broad range of cell types (see figures "High Transfection Efficiencies Using Effectene Reagent" and "Transfection of Oligonucleotides Using Effectene Reagent"). Because transfection can be performed in the presence of serum and requires low amounts of DNA (see figure "Serum and DNA Quantity vs. Transfection Efficiency"), cytotoxicity is minimal. This makes Effectene Reagent particularly effective for primary cells (see figure "Effectene Reagent with Primary Cells"). A searchable list of cell lines and primary cells successfully transfected using Effectene Reagent, as well as customer-developed transfection protocols, is available at the Transfection Tools web site — www.qiagen.com/TransfectionTools. View cell lines successfully transfected with this product.

Effectene Transfection Reagent  effectene轉(zhuǎn)染試劑

High-throughput transfection

The application of recombinant DNA technology to fields such as drug discovery and development has led to an increased need for high-throughput transfection. Transfection using Effectene Reagent requires low amounts of DNA and minimal handling, and in addition, removal of transfection complexes is not required, making this reagent highly suitable for high-throughput screening. Effectene Reagent provides outstanding transfection efficiencies, excellent reproducibility, and minimal cytotoxicity in high-throughput transfection, and is available in bulk quantities.

Cited References

1. Ausubel, F.M., et al. eds. (1991) Current Protocols in Molecular Biology, Wiley Interscience, New York.
2. Sambrook, J., Fritsch, E.F., and Maniatis, T., eds. (1989) Molecular cloning — a laboratory manual, 2nd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor.

Images

Model of Effectene Principle High Transfection Efficiencies Using Effectene Reagent Transfection of Oligonucleotides Using Effectene Reagent Effectene Transfection Procedure Serum and DNA Quantity vs. Transfection Efficiency Effectene Reagent with Primary Cells

 

Effectene Transfection Reagent  effectene轉(zhuǎn)染試劑 詳細(xì)產(chǎn)品信息可和選購(gòu)


留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
西西444www无码大胆| 成人网站在线观看| 男主骗女主帮她发育h| 国模冰冰大胆瓣开下部| 国产美女久久精品香蕉69| 玩弄了裸睡少妇人妻野战| 男人狂躁进女人免费视频| 国产精品久久毛片| 亚洲国产精品国自产拍AV| 最新国产の精品合集bt7086 | 国产肥熟女视频一区二区三区| 亚洲性爱视频| 黄色视频app下载| 国内老熟妇对白HDXXXX| 欧美成人看片一区二区三区尤物| 性色av无码| 学长别揉了我快尿了男男| 久久久久久久久波多野高潮| 无遮挡国产高潮视频免费观看 | 久久精品国产亚洲AV高清色欲| 国产一区二区三区小说| 性欧美大战久久久久久久久| 人善交videos欧美| 国产成人精品999在线观看 | 国产欧美日韩丝袜高跟鞋| 男人用嘴添女人下身免费视频| 把腿张开被舔得死去活来在线| 7777色鬼XXXX欧美色妇| 久久久久久精品免费无码无| 国产熟女露脸大叫高潮| 多p混交群体交乱嗯啊小说| 免费国偷自产拍精品视频| 亚洲 成人 综合 另类 图区| 国产人妻777人伦精品hd| 老人玩小处雌女视频| 亚洲欧美国产精品专区久久| 久久 国产 尿 小便 嘘嘘| 色综合久久久久久久久五月| 亚洲国产天堂久久综合| 中文字幕AⅤ人妻一区二区| 99久久免费国产精品|